|
KMID : 1234420120400020098
|
|
Korean Journal of Microbiololgy and Biotechnology
2012 Volume.40 No. 2 p.98 ~ p.103
|
|
|
The Isolation and Culture Characterization of a Lipolytic Enzyme Producing Strain from Meju
|
|
Yun Hye-Ju
Lee You-Jung
Yeo Soo-Hwan
Choi Hye-Sun
Park Hye-Young
Baek Seong-Yeol
Park Heui-Dong
|
|
|
Abstract
|
|
|
|
For screening of useful enzymes producing microorganisms from Meju, we isolated high lipase producing strains and their lipolytic enzyme activities were then tested. The lipolytic enzyme activities of isolated microorganisms were therefore tested on the Y124 strain. The gene sequence analysis of ITS from Y124 strain revealed Yarrowia lipolytica. Lipase production by the Y124 strain was studied in media containing various carbon sources. The Y124 strain drastically increased lipolytic enzyme activity in YPO media containing olive oil, as well as in YPDO media containing both olive oil and glucose. Maximal lipase production was achieved in YPD (yeast extract-peptone-D-glucose) media containing 0.7% olive oil when cultured at 30oC for 8 hrs. The lipase produced from the Y124 strain showed the highest activity in p-NPO (p-nitrophenyl octanoate (C8)), amongst the various p-nitrophenyl esters.
|
|
|
KEYWORD
|
|
|
Yarrowia lipolytica, 16S rDNA, Lipase activity, carbon source
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|